ABSTRACT
To determine the prevalence spectrum of methicillin resistant Staphylococcus epidermidis in biomaterial infections. Experimental and observational study. This study was conducted in the department of Microbiology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, during the period of January 2010 to Dec. 2010. A total of 300 subjects of all ages and sex were included. Swab from cannulae tips, catheters tips, old cannulae infected wounds, injection abscess were collected from Jinnah Postgraduate Medical Centre, National Institute of Child Health and Civil Hospital, Karachi and processed according to standard laboratory methods. A total 103 methicillin resistant Staphylococcus epidermidis, causing biomaterial related infection, isolated from 300 patients were analyzed. Bacterial pathogens were commonly isolated from patients of all ages who developed biomaterial related infections. Patients suffering from infections related with biomaterial should be monitored for MRSE at regular intervals
ABSTRACT
To assess bacteriological spectrum, of isolates from cases of nosocomial infections in pediatric ICU. subjective study that was carried out from November 2009 to September 2010, on two hundred samples which were taken from patients in pediatric ICU, tertiary care hospital who were clinically suspected of having nosocomial infection and processed for the diagnosis and isolation of the infective organisms in the Department of Microbiology, Basic Medical Sciences Institute [BMSI], Jinnah Postgraduate Medical Centre [JPMC], Total 200 samples were collected. Out of these 143 samples showed positive results. 138 were of bacterial growth while 5 samples were positive for fungus. Among these 61 were positive for septicemia, 44 positive for UTI and 17 samples were positive for respiratory tract related disease, while 21 positive for miscellaneous samples. This study presents a general overview of nosocomial pathogens in Pediatric ICUS and emphasizes the importance of timely clinical and bacteriological monitoring among children especially in patients in critically ill situation
Subject(s)
Humans , Male , Female , Intensive Care Units, Pediatric , Tertiary Care CentersABSTRACT
The objective of the study was to identify the species of enterobacteriaceae from the post operative patients and their susceptibility pattern. Rising antibiotic resistance rates among bacterial pathogens has resulted in increased morbidity and mortality. In Pakistan lack of infection control practice and non-existence of formal antibiotics policies has further augmented this problem. 200 postoperative wounds swabs were taken from different surgical and gynaeccolgy/obstetric wards of hospitals, specimens were then inoculated on Blood agar and MacConkey agar medium. The Enterobacteriaceac were identified by using battery of tests. Antimicrobial susceptibility was determined by Kirby Bauer disc diffusion method as per CLSI. E. coli isolates showed 100% resistance to ampicillin, while imepenem showed 94.3% sensitivity. Klebsiella species were found 100% resistant to ampicillin and 96.8% sensitive to imepenem. Enterobacteriaceae isolates show nearly the same type of multi-drug resistance even though some species showed 100% resistance to some antibiotics